Direct surface-enhanced Raman scattering (SERS) spectroscopy of nucleic acids: from fundamental studies to real-life applications. Gold Nanorods as Surface-Enhanced Raman Spectroscopy Substrates for Rapid and Sensitive Analysis of Allura Red and Sunset Yellow in Beverages. Surface-Enhanced Raman Spectroscopy. Recent Advancement of Raman Spectroscopy. Ujjal Kumar Sur. Keywords. Raman scattering, surface en-.
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Surface enhanced Raman scattering SERS is a technique which offers orders of magnitude increases in Raman intensity, overcoming the traditional drawback of Raman scattering — its inherent weakness.
Enhancement factors can be as high aswhich are sufficient to allow even single molecule detection using Raman. Using antibodies and gold particles this approach can quantify proteins in serum with high sensitivity and specificity.
Specific single nucleotide polymorphisms SNP surface enhanced raman scattering be identified using this technique.
This has several potential applications: For example, Cao et al. Each spectrum was specific, which is advantageous over fluorescence detection; some fluorescent markers overlap and interfere with other gene markers.
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- Surface-enhanced Raman spectroscopy
- Raman Spectroscopy
The advantage of this technique to identify gene sequences is that several Raman dyes are commercially available, which could lead to the development of non-overlapping probes for gene detection.
While the spectra of most SERS experiments are similar to the non-surface enhanced spectra, there are often differences in the number of modes present. Additional modes not surface enhanced raman scattering in the traditional Raman spectrum can be present in the SERS spectrum, while other modes can disappear.
The modes observed in any spectroscopic experiment are dictated by the symmetry of the molecules and are usually summarized by selection rules. When molecules are adsorbed to a surface, the symmetry of the system can change, slightly modifying the symmetry of the molecule, which can lead to differences in mode selection.
The loss surface enhanced raman scattering a center of symmetry eliminates the requirements surface enhanced raman scattering the mutual exclusion rulewhich dictates that modes can only be either Raman or Infrared active.
Thus modes that would normally appear only in the infrared spectrum of the free molecule can appear in the SERS spectrum. Reproduced material should be attributed as follows: For reproduction of material from Surface enhanced raman scattering For reproduction of material from PCCP: For reproduction of material from PPS: